Recessive suppressors in Aspergillus nidulans closely linked to an auxotrophic mutant which they suppress

Isolating Auxotrophic Mutants in Aspergillus nidulans

A method for the selection of auxotrophic mutants in Aspergillus nidulans using N-glycosyl-polifungin has been developed. The method results in about 9000fold enrichment. All types of auxotrophs were obtained. Under certain conditions preferential selection of nucleic acid hydrolysate-requiring auxotrophs was observed. The frequency of occurrence of these mutants was about 50 % of all colonies ...

The mechanism of action of proline suppressors in Aspergillus nidulans.

Asexual recombination in a uvsH mutant of Aspergillus nidulans.

Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency ...

Wild-type and mutant stocks of Aspergillus nidulans.

VER the past decade the homothallic ascomycete Aspergillus nidulans has become an important organism for genetic investigation. As a consequence of the pioneering research of PROFESSOR G. PONTECORVO and associates, a wide variety of mutant strains have become available for research and teaching. Recognizing these facts, the Committee on the Maintenance of Genetic Stocks of the Genetics Society ...

Aspergillus nidulans

Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...